Genetic diversity in melissa officinalis accessions by. Jaccards coefficient was also used to analyze the presenceabsence of data. Genetic diversity is the base for survival of plants in nature and for crop improvement. Numerical taxonomy and multivariate analysis system. Allelic polymorphic information content pic was calculated using the formula pic 1 p ij2,wherep ij is the frequency of the ith allele in the jth population, for each ssr locus botstein et al.
Udo 39 to 3 gapu 59, dca 09 and dca 18 with a mean value of 3. Twosample t tests devore and peck 1997 were performed to determine if the average genetic distances between ss and nss heterotic groups were statistically significant. Traditional breeding efforts made to introgress complex traits like salinity tolerance have met with limited success and only a few salttolerant varieties 30 cultivars in 12 different plant species have been developed and released for. Sago palm pollination may occur as selfpollinated and crosspollinated. Results a total of 68 alleles were detected across all eight ssr. Isolation and characterization of plant growth promoting. Mornings were devoted to lectures in the, more or less, theoretical aspects while afternoons were, generally, used by the delegates to practice mostly with their own specimens image acquisition, landmark capture and become familiar with james thin plate spline software, ntsys and other packages for morphometric analysis. Results and discussion overall microsatellite diversity a total of 37 alleles were observed across the ten markers, the number of alleles per locus ranging from 5 udo 28. A total of 22 endophytic bacteria were isolated from roots and nodules of vigna radiata mungbean obtained from jind district, haryana.
Ntsys pc rohlf, 1996 to calculate an unweighted paired cage and intercrossed. Genetic variability in a brazilian apple germplasm. Random amplified polymorphic dna markers alsike clover at least 40 seeds per clover population were germinated auroraisawinterhardycultivar developedinwesterncan in the greenhouse. The program originated as ntsys in the 1960s but over the additional information about new features in version 2. Rapd primers ubc 5 cct ggg ttc c ubc 6 cct ggg cct a ubc 23 ccc gcc ttc a ubc 30 the utility of the rapd markers in identifying chickpea ccg gcc tta g. Genetic variation of rapd markers for north american white. Rohlf and others published ntsyspc numerical taxonomy and multivariate. Boxpcr and ericpcrbased genotyping and phylogenetic. Rapd analysis reveals genetic variability among sexual and. Results and discussion based on parental survey analysis using 50 ssr different chromosomes of maize genome, were found highly polymorphic for both parents and hybrids of cv. Reiss commonly known as espinheira santa and their antimicrobial properties were investigated. The astonishing spectrum of scarabaeine lifestyles makes them an attractive group for studies in entomology and evolutionary biology. Paspalum dilatatum is a valuable forage grass in the subtropics. Genetic diversity and relationship of hedychium from northeast.
A binary data matrix based on presenceabsence was used for analysis with ntsyspc software package rohlf 1997. The polymorphism information content pic value was calculated as pic. Results protein analysis the patterns of hmw glutenin subunits among 28 populations of jointed goatgrass detected that diversity in the threeband hmw glutenin subunits pattern was extremely low. Results and discussion neighbor joining analysis provided the most logical arrangement of cultivar relationships. Similarity between accessions was estimated using dice coefficient. Pdf ntsyspc numerical taxonomy and multivariate analysis. The cluster analysis was performed based on protein bands using weighted. St distances using the software package ntsys rohlf 1998. This species consists of several sexual tetraploid and apomict penta and hexaploid biotypes. Ntsys merupakan program yang dibuat untuk melakukan analisis kekerabatan. Ntsys software was used to determine the genetic similarities and construct a dendrogram.
Ntsyspc is one of the most popular softwares being used in molecular genetic qualitative data. Evaluation of hbrmite markers for assessment of genetic. Only clear and reproducible fragments were scored and the data matrices of the issr fragments were accumulated for further investigation. Collaborative filtering or recommender systems use a database about user preferences to predict additional topics or products a new user might like. Frontiers cultivated olive diversification at local and. A basic statistical package designed to be used with the fourth edition of the text biometry by sokal and rohlf. In this paper we describe several algorithms designed for this task, including techniques based on correlation coefficients, vectorbased similarity calculations, and statistical bayesian methods. Detection of genetic variation in sandalwood using various. Numerical taxonomy and multivariate analysis system version 2.
Although, large collection has been established in genebank, there is a serious concern over its management, conservation and utilization because of limited national funding. The morphological qualitative data was scored numerically as present 1 and absent 0, and then analyzed using ntsys pc rohlf, 1998. Genetic diversity and classification of 51 strains of. Genetic diversity of hibiscus tiliaceus malvaceae in. Differences in levels of heterozygosity in populations of. Rohlf, 1998 results dna polymorphism among genotypes a varying level of genetic polymorphism was revealed in the banding pattern across 45 chilli accessions figure 1.
Genetic diversity as revealed by intersimple sequence. Genetic diversity of pear pyrus spp germplasm assessed. As a result of adaptions to specific food substrates and textures, the mouthparts of dung beetles, particularly the mandible, have undergone considerable evolutionary changes and differ distinctly from the presumptive ancestral conditions of the coleoptera. A binary matrix containing only distinct french genotypes was built, using alleles scored as present 1 or absent 0 to assess genetic relationships within the fogb collection. The rapd and issr products were scored as presence 1 or absence of band 0 in all samples for each primer genotype combination. These data were applied to generate a similarity matrix based on jaccards coefficients by using the simqual program in the ntsys pc software package version 2. Input is through imported ntsyspcformatted excel files and results appear in an output. Genetic diversity in 20 elite blackgram vigna mungo l. Collection, characterization and conservation of taro. Deciphering the evolutionary affiliations among bacterial. Genetic diversity and biogeography of the traditional. Similarity coefficients for pairs of isolates were calculated using the ntsys 2. Numerical taxonomic study of the genus aedes of america north of mexico abstract. A dendrogram was constructed using the unweighted pairgroup arithmetic averaging clustering method with the sequential agglomerative hierarchical nesting algorithm.
Deciphering the evolutionary affiliations among bacterial strains pseudomonas and frankia sp. Population genetic structure based on ssr markers in. Sago palm is flowering and fruiting just once in their life cycle. A total of 34 pear accessions, derived presumably from at least six species, were subjected to simple sequence repeat ssr analysis. The wild species progenitors of these landraces have long. The matrix correlation of geographical and genetic distances mantel, 1967 used ntsys pc 2. Finally, dendrogram was construct by sahn upgma clustering. Duplicate measurements for each specimen were averaged and were used to design a data matrix of pairwise similarities between genotypes lines.
Sago palms that grow naturally and semi cultivated were generally occurred natural pollination to form fruits and seeds, if not cut down to take the starch contained in their trunk. Relationship of european persimmon diospyros kaki thunb. It has been proposed that the presence of a genome of unknown origin, the x genome, is responsible for apomixis in penta and hexaploid biotypes. Thirteen endophytic fungal strains of the genus pestalotiopsis were isolated from the medicinal plant maytenus ilicifolia mart.
Plant breeding symposium of the southern african plant breeders association, golden gate, south africa, 1719 march 1998. Upgma unweighted pairgroup method using arithmetic average was performed from the shan option and a dendrogram was derived from the tree option. This matrix was used to construct a dendrogram based on dices similarity index and the upgma algorithm with the ntsys v2. Ntsys pc numerical taxonomy and multivariate analysis system, version 2. Deciphering the evolutionary affiliations among bacterial strains. The apple malus domestica borkh originally evolved to require temperatures below 7. The cultivated potato, solanum tuberosum, ultimately traces its origin to andean and chilean landraces developed by precolombian cultivators. Transferability of microsatellite markers across eleven.
Diversity in plant genetic resources provides opportunity for plant breeders to develop new and improved cultivars with desirable characteristics, which include both farmerpreferred traits high yield potential, large seed, etc. A read is counted each time someone views a publication summary such as the title, abstract, and list of authors, clicks on a figure, or views or downloads the fulltext. Classification of pure lines of germplasm based on larval durations and determination of relationships amount of these pure lines are necessary in order to carry out successfully any silkworm breeding project. Its wide geographical distribution and varied habitats indicate that there is probably a large amount of genetic diversity. Each stream variable showed a particular pattern of variation throughout the study area.
Housui and yali cultivars, example of pyrus pyrifolia and p. Numerical taxonomy and multivariate analysis system, version 2. A new technique in the preserving of softbodied insects and spiders. Software programs for analysing genetic diversity nk. Microsatellite markerbased identification and genetic. Unequivocally reproducible bands were scored and entered into a binary character matrix 1 for presence and 0 for absence. All statistical analysis was performed with aid ntsys pc computer genotypes with cluster analysis using the shan module of ntsy sp. The pairwise genetic similarity coefficient gs was calculated using jaccard coefficient jaccard 1908 by the simqual program of ntsys pc software version 2. The matrix of average taxonomic distance was estimated using. Hibiscus tiliaceus is sometimes cultivated inland for home landscapes as an ornamental shade tree. These cultivars are grown in many warmer climate countries in south america.
Genetic variation analysis of mold magnaporthe oryzae b. These selected markers were used for testing seed genetic purity. The data were subjected to hierarchical cluster analysis hca and principal component analysis pca using ntsys rohlf, 1998. Assessment of genetic diversity among capsicum annuum l.
In the present study, the abb germplasm was characterised through morphotaxonomic traits and molecular markers, with aa and bb genomes included as reference groups. Upgma unweighted pairgroup method using arithmetic averages cluster analysis and a multidimensional scaling analysis mds were also performed on the data set with ntsys 2. Genetic variation of sago palm metroxylonsagu rottb. This study was conducted to identify and recognize relations and diversity among accessions of the iranianpears pyrus spp.
These andean landraces exhibit tremendous morphological and genetic diversity, and are distributed throughout the andes, from western venezuela to northern argentina, and in southern chile. Environmental heterogeneity explains the genetic structure of continental and mediterranean populations of fraxinus angustifolia vahl. Hepper genotypes was studied using microsatellite and aflp markers. Dalam biologi molekuler, data binomial digunakan dalam rapd dan juga aflp. User guide find, read and cite all the research you need on researchgate.
Two isolates were successful in inhibiting the growth of the tested microorganisms escherichia coli, klebsiella pneumoniae, micrococcus luteus, staphylococcus. Fungal biotechnology in agricultural, food, and environmental applications. Average taxonomic distance was used to group the quantitative data using dist coefficient. Bioprospecting highly diverse endophytic pestalotiopsis. Program ini dapat digunakan untuk melihat hubungan kekerabatan antara beberapa sampel spesies dengan melihat muncultidaknya suatu parameterfaktor fisik pada masingmasing sampel. Jaccards similarity coefficient was estimated using ntsys 2. Thirtysix microsatellite markers from azuki bean vigna angularis willd. Rust susceptibility of important south african commercial dry bean cultivars, and possible sources of resistance. A single domestication for potato based on multilocus. The simple matching coefficient was used to measure similarity, as it was the coefficient with the best results following a cophenetic test. Read genetic diversity of bitter gourd momordica charantia l. Most of the cultivated rice varieties are susceptible to salinity but rice germplasm do have sources for salt tolerance trait flowers et al. Ntsys pc numerical taxonomy and multiware analysis system rohlf, 1988.
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